Hotspot

trnH-GUG-psbA

LSC · 69-407 · 339 bp

Back to Symphoricarpos

Pi 0

MarkerSeek score 34.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTCTAGACCTAGCCGCTCTT	55.0	55.0	58.1	58.0	405 bp	1.0	78.8
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CTCTAGACCTAGCCGCTCTT	50.0	55.0	57.9	58.0	419 bp	1.0	78.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CTCTAGACCTAGCCGCTCTT	50.0	55.0	57.9	58.0	418 bp	1.0	78.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CTCTAGACCTAGCCGCTCTT	50.0	55.0	57.9	58.0	420 bp	1.0	78.7
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TTTCCCTCTAGACCTAGCCG	55.0	55.0	58.1	57.9	410 bp	1.0	78.4