Hotspot

rpoC1

LSC · 22173-24960 · 2788 bp

Back to Sycopsis

Pi 0.00215

MarkerSeek score 57.30

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2788
variable_sites: 6
pi: 0.00215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GAGAGGACCGAATTCACACC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.0	58.0	2962-2963 bp	1.0	79.7
rpoC1_p2	GGAGAGGACCGAATTCACAC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.0	58.0	2963-2964 bp	1.0	79.7
rpoC1_p3	CCTAGTTGAACCGGAGAGGA	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.2	58.0	2975-2976 bp	1.0	78.6
rpoC1_p4	GGACCGAATTCACACCGTAT	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.7	58.0	2958-2959 bp	1.0	77.4
rpoC1_p5	GAGAGGACCGAATTCACACC	GCTCGTTCGAGAACTACGAT	55.0	50.0	58.0	57.5	2960-2961 bp	1.0	76.4