Hotspot

matK

LSC · 2038-3552 · 1515 bp

Back to Sycopsis

Pi 0.00264

MarkerSeek score 60.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 4
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACTGCATGAACATTTCAGAATCG	AGTTTACCTGTCTCCGAGGT	39.1	50.0	58.0	57.7	1722 bp	1.0	53.6
matK_p2	CTGCATGAACATTTCAGAATCGA	AGTTTACCTGTCTCCGAGGT	39.1	50.0	57.8	57.7	1721 bp	1.0	51.9
matK_p3	TCAACCTTTACTGCATGAACA	AGTTTACCTGTCTCCGAGGT	38.1	50.0	55.7	57.7	1731 bp	1.0	51.5
matK_p4	TGCATGAACATTTCAGAATCGA	AGTTTACCTGTCTCCGAGGT	36.4	50.0	56.7	57.7	1720 bp	1.0	51.4
matK_p5	CAGTTGATTCAACCTTTACTGCA	AGTTTACCTGTCTCCGAGGT	39.1	50.0	57.6	57.7	1739 bp	1.0	50.2