Hotspot

ndhD-psaC

SSC · 121547-121656 · 110 bp

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Pi 0.0182

MarkerSeek score 63.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 110
variable_sites: 2
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAAATTAAGGAACCCGCGGA	AGAGGACTGTGTCGGTTGTA	45.0	50.0	57.8	58.0	305-306 bp	1.0	78.8
ndhD-psaC_p2	AAATTAAGGAACCCGCGGAT	AGAGGACTGTGTCGGTTGTA	45.0	50.0	57.6	58.0	304-305 bp	1.0	77.0
ndhD-psaC_p3	CGCGGATATCGGCAAAACTA	AGAGGACTGTGTCGGTTGTA	50.0	50.0	58.5	58.0	291-292 bp	1.0	76.4
ndhD-psaC_p4	AAAATTAAGGAACCCGCGGA	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.8	57.7	286-287 bp	1.0	76.4
ndhD-psaC_p5	GAAAATTAAGGAACCCGCGG	AGAGGACTGTGTCGGTTGTA	50.0	50.0	57.4	58.0	306-307 bp	1.0	76.0