Hotspot

rpl32-trnL-UAG

SSC · 117537-118595 · 1059 bp

Back to Sycopsis

Pi 0.00573
MarkerSeek score 50.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1059
variable_sites
6
pi
0.00573
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTGTGCCGACAAATACAAA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 54.3 57.9 1122-1125 bp 1.0 50.8
rpl32-trnL-UAG_p2 TTTGTGCCGACAAATACAAA AGCAGCGTGTCTACCAATTT 35.0 45.0 54.3 57.8 1115-1118 bp 1.0 50.4
rpl32-trnL-UAG_p3 TTTGTGCCGACAAATACAAA CCTAAGAGCAGCGTGTCTAC 35.0 55.0 54.3 57.8 1121-1124 bp 1.0 50.3
rpl32-trnL-UAG_p4 TTTGTGCCGACAAATACAAA CTAAGAGCAGCGTGTCTACC 35.0 55.0 54.3 57.8 1120-1123 bp 1.0 50.3
rpl32-trnL-UAG_p5 TTTGTGCCGACAAATACAAA GAGCAGCGTGTCTACCAATT 35.0 50.0 54.3 58.3 1116-1119 bp 1.0 49.7