Hotspot

petD-rpoA

LSC · 81283-81470 · 188 bp

Back to Sycopsis

Pi 0.011

MarkerSeek score 61.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 2
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CATTTCGTCGTCCAGTAGCA	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.0	55.2	351-358 bp	1.0	59.7
petD-rpoA_p2	GGTTGGGTATTGGAGCAACA	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.1	55.2	294-301 bp	1.0	59.6
petD-rpoA_p3	TGGTTGGGTATTGGAGCAAC	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.1	55.2	295-302 bp	1.0	59.5
petD-rpoA_p4	CCAGTAGCAACAACCGTCTT	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.1	55.2	340-347 bp	1.0	58.9
petD-rpoA_p5	TTTCGTCGTCCAGTAGCAAC	TTGGGCATTCTAGAAAAGCA	50.0	40.0	58.2	55.2	349-356 bp	1.0	58.0