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psaJ-rpl33

LSC · 70739-71167 · 429 bp

Back to Sycopsis

Pi 0.00466

MarkerSeek score 44.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 2
pi: 0.00466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACCCGTGACATTTTGTACCC	CTGTTCCTTGGAAGGGTGAC	50.0	55.0	58.1	58.1	1246-1250 bp	1.0	78.7
psaJ-rpl33_p2	CCCGTGACATTTTGTACCCA	CTGTTCCTTGGAAGGGTGAC	50.0	55.0	58.1	58.1	1245-1249 bp	1.0	78.7
psaJ-rpl33_p3	ACAGGATTTGAACCCGTGAC	CTGTTCCTTGGAAGGGTGAC	50.0	55.0	58.1	58.1	1257-1261 bp	1.0	78.6
psaJ-rpl33_p4	CAGGATTTGAACCCGTGACA	CTGTTCCTTGGAAGGGTGAC	50.0	55.0	58.1	58.1	1256-1260 bp	1.0	78.6
psaJ-rpl33_p5	TGACAGGATTTGAACCCGTG	CTGTTCCTTGGAAGGGTGAC	50.0	55.0	58.1	58.1	1259-1263 bp	1.0	78.6