Hotspot

atpB-rbcL

LSC · 57785-58525 · 741 bp

Back to Sycopsis

Pi 0.00405

MarkerSeek score 61.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 3
pi: 0.00405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	878-887 bp	1.0	72.6
atpB-rbcL_p2	GGGGGAAAAGCTACATCCAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	891-900 bp	1.0	72.5
atpB-rbcL_p3	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	877-886 bp	1.0	72.3
atpB-rbcL_p4	GGGGGAAAAGCTACATCCAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	890-899 bp	1.0	72.2
atpB-rbcL_p5	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	876-885 bp	1.0	71.9