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petN-psbM

LSC · 30354-31535 · 1182 bp

Back to Sycopsis

Pi 0.00254

MarkerSeek score 60.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1182
variable_sites: 3
pi: 0.00254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.1	57.6	1304-1306 bp	1.0	68.2
petN-psbM_p2	CCCTTTCGCTCGTAGTATGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	1297-1299 bp	1.0	68.0
petN-psbM_p3	CCTTTCGCTCGTAGTATGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	1296-1298 bp	1.0	68.0
petN-psbM_p4	CTTTCGCTCGTAGTATGGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	1295-1297 bp	1.0	68.0
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1342-1344 bp	1.0	67.8