Hotspot

rpoB-trnC-GCA

LSC · 28200-29345 · 1146 bp

Back to Sycopsis

Pi 0.00699

MarkerSeek score 62.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1146
variable_sites: 8
pi: 0.00699
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCTGATCAATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1303-1304 bp	1.0	79.7
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1285-1286 bp	1.0	77.5
rpoB-trnC-GCA_p3	AAAGTTCTTCCGTCAAGCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1313-1314 bp	1.0	76.7
rpoB-trnC-GCA_p4	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1233-1234 bp	1.0	74.6
rpoB-trnC-GCA_p5	AAAGTTCTTCCGTCAAGCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1295-1296 bp	1.0	74.6