Hotspot

rps2-rpoC2

LSC · 17604-17844 · 241 bp

Back to Sycopsis

Pi 0.029

MarkerSeek score 69.20

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 7
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	ACCAAAATGAACCCCTGCTT	TTCGAGGGGAAAATGAGAGA	45.0	45.0	57.9	55.9	421 bp	1.0	63.4
rps2-rpoC2_p2	AATGAACCCCTGCTTCCATC	TTCGAGGGGAAAATGAGAGA	50.0	45.0	57.9	55.9	416 bp	1.0	63.0
rps2-rpoC2_p3	GAACCCCTGCTTCCATCATC	TTCGAGGGGAAAATGAGAGA	55.0	45.0	58.3	55.9	413 bp	1.0	61.7
rps2-rpoC2_p4	ACCAAAATGAACCCCTGCTT	TCGAGGGGAAAATGAGAGAT	45.0	45.0	57.9	55.6	420 bp	1.0	61.5
rps2-rpoC2_p5	GACCAAAATGAACCCCTGCT	TTCGAGGGGAAAATGAGAGA	50.0	45.0	58.4	55.9	422 bp	1.0	61.3