Hotspot

ycf1

SSC · 124154-129751 · 5598 bp

Back to Syagrus

Pi 0.00241

MarkerSeek score 54.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5598
variable_sites: 26
pi: 0.00241
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGAAAATCCGATTGTTGCG	AAAAGGTTCCTCGATGGTCA	45.0	45.0	57.8	56.8	236 bp	1.0	70.2
ycf1_p2	GCGTATCAAAGCCACCTCTT	AAAAGGTTCCTCGATGGTCA	50.0	45.0	58.3	56.8	212 bp	1.0	70.2
ycf1_p3	GTAGCGTATCAAAGCCACCT	AAAAGGTTCCTCGATGGTCA	50.0	45.0	57.7	56.8	215 bp	1.0	69.8
ycf1_p4	ACGAAAATCCGATTGTTGCG	AAAAAGGTTCCTCGATGGTCA	45.0	42.9	57.8	57.5	237 bp	1.0	68.1
ycf1_p5	GCGTATCAAAGCCACCTCTT	AAAAAGGTTCCTCGATGGTCA	50.0	42.9	58.3	57.5	213 bp	1.0	68.1