Hotspot

rpoC1

LSC · 21238-24036 · 2799 bp

Back to Syagrus

Pi 0.00113

MarkerSeek score 56.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2799
variable_sites: 6
pi: 0.00113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TGAAACTGGAGAGACCCGAA	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.3	58.0	2978-2980 bp	1.0	77.6
rpoC1_p2	GGAGAGACCCGAATCCCTAC	TTGCTCGTTCGAGAACTACG	60.0	50.0	58.7	58.0	2971-2973 bp	1.0	74.5
rpoC1_p3	TGAAACTGGAGAGACCCGAA	GCTCGTTCGAGAACTACGAT	50.0	50.0	58.3	57.5	2976-2978 bp	1.0	74.2
rpoC1_p4	AACTGGAGAGACCCGAATCC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.8	58.0	2975-2977 bp	1.0	73.4
rpoC1_p5	GTGAAACTGGAGAGACCCGA	TTGCTCGTTCGAGAACTACG	55.0	50.0	59.0	58.0	2979-2981 bp	1.0	71.6