Hotspot

trnH-GUG-rps19

IRa · 154600-154803 · 204 bp

Back to Syagrus

Pi 0.00245
MarkerSeek score 33.10
Primer available yes
Label trnH-GUG-rps19

Feature Metadata

label_name
trnH-GUG-rps19
feature_type
igs
region
IRa
length_bp
204
variable_sites
1
pi
0.00245
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-rps19_p1 GATCCACTTGGCTACATCCG TGCCCGAGACCAAGTTACTA 55.0 50.0 58.1 58.1 267 bp 1.0 78.7
trnH-GUG-rps19_p2 CAATCCACTGCCTTGATCCA TGCCCGAGACCAAGTTACTA 50.0 50.0 57.9 58.1 281 bp 1.0 78.6
trnH-GUG-rps19_p3 AATCCACTGCCTTGATCCAC TGCCCGAGACCAAGTTACTA 50.0 50.0 57.9 58.1 280 bp 1.0 78.6
trnH-GUG-rps19_p4 ACAATCCACTGCCTTGATCC TGCCCGAGACCAAGTTACTA 50.0 50.0 57.9 58.1 282 bp 1.0 78.6
trnH-GUG-rps19_p5 TAATGTGATGGGCGAACGAC TGCCCGAGACCAAGTTACTA 50.0 50.0 58.4 58.1 330 bp 1.0 76.8