Hotspot

ndhH-rps15

SSC · 123374-123486 · 113 bp

Back to Syagrus

Pi 0.00885

MarkerSeek score 59.60

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 113
variable_sites: 2
pi: 0.00885
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	AACACCATGCATTGATGGGT	GGCTCCTGGCTTATTTGTCA	45.0	50.0	58.1	57.9	276-277 bp	1.0	79.4
ndhH-rps15_p2	TCAGTCGAAGAACACCATGC	GGCTCCTGGCTTATTTGTCA	50.0	50.0	58.2	57.9	286-287 bp	1.0	78.2
ndhH-rps15_p3	ATGCATTGATGGGTGGTGAG	GGCTCCTGGCTTATTTGTCA	50.0	50.0	58.2	57.9	270-271 bp	1.0	78.0
ndhH-rps15_p4	AACACCATGCATTGATGGGT	AACGGCTCCTGGCTTATTTG	45.0	50.0	58.1	58.5	279-280 bp	1.0	76.1
ndhH-rps15_p5	AACACCATGCATTGATGGGT	CAACGGCTCCTGGCTTATTT	45.0	50.0	58.1	58.5	280-281 bp	1.0	76.1