Hotspot

psaC-ndhE

SSC · 117411-118027 · 617 bp

Back to Syagrus

Pi 0.00783
MarkerSeek score 64.10
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
617
variable_sites
9
pi
0.00783
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA CTGAAGCAGCTATTGGGCTA 50.0 50.0 58.1 57.4 811-812 bp 1.0 74.9
psaC-ndhE_p2 ACATCCGTCCCAAGGTATCA TGAAGCAGCTATTGGGCTAG 50.0 50.0 58.1 57.4 810-811 bp 1.0 74.9
psaC-ndhE_p3 ACATCCGTCCCAAGGTATCA TGTTATAGCTGTTGCGGCTG 50.0 50.0 58.1 58.6 831-832 bp 1.0 74.8
psaC-ndhE_p4 ACATCCGTCCCAAGGTATCA GAAGCAGCTATTGGGCTAGC 50.0 55.0 58.1 58.8 809-810 bp 1.0 73.8
psaC-ndhE_p5 CAATACATCTGTGGGGCAGG TGAAGCAGCTATTGGGCTAG 55.0 50.0 58.3 57.4 786-787 bp 1.0 73.4