Hotspot

rpl20-rps12

LSC · 68984-69662 · 679 bp

Back to Syagrus

Pi 0.00172

MarkerSeek score 60.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 2
pi: 0.00172
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CGACGTTTCCGAGCTGTATA	TCTCCCGCTCTTAGAGGATG	50.0	55.0	57.5	58.0	806-807 bp	1.0	76.5
rpl20-rps12_p2	CGACGTTTCCGAGCTGTATA	TGTATGTGCGACTCGTTCAG	50.0	50.0	57.5	58.0	757-758 bp	1.0	76.4
rpl20-rps12_p3	CGACGTTTCCGAGCTGTATA	GAGGAACATGCACTAGGGTG	50.0	55.0	57.5	58.0	775-776 bp	1.0	76.4
rpl20-rps12_p4	CGACGTTTCCGAGCTGTATA	GTCGAGGAACATGCACTAGG	50.0	55.0	57.5	58.1	778-779 bp	1.0	76.0
rpl20-rps12_p5	CGACGTTTCCGAGCTGTATA	CACTAGGGTGTATGTGCGAC	50.0	55.0	57.5	58.1	765-766 bp	1.0	76.0