Hotspot

trnS-GCU-trnG-UCU; trnG-UCC

LSC · 8254-9336 · 1083 bp

Back to Syagrus

Pi 0.00446

MarkerSeek score 59.90

Primer available yes

Label trnS-GCU-trnG-UCU; trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCU; trnG-UCC
feature_type: igs
region: LSC
length_bp: 1083
variable_sites: 9
pi: 0.00446
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCU; trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	1157-1200 bp	1.0	52.3
trnS-GCU-trnG-UCU; trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	1160-1203 bp	1.0	51.2
trnS-GCU-trnG-UCU; trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	1159-1202 bp	1.0	51.2
trnS-GCU-trnG-UCU; trnG-UCC_p4	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	1213-1256 bp	1.0	50.7
trnS-GCU-trnG-UCU; trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	1158-1201 bp	1.0	50.4