Hotspot

trnK-UUU-rps16

LSC · 3985-4698 · 714 bp

Back to Syagrus

Pi 0.00794
MarkerSeek score 64.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
714
variable_sites
11
pi
0.00794
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCTCAACCTACGGGAACTG 50.0 57.9 58.2 57.9 829-836 bp 1.0 70.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GCTCAACCTACGGGAACTG 50.0 57.9 58.2 57.9 828-835 bp 1.0 70.6
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GTGCTCAACCTACGGGAAC 50.0 57.9 58.2 58.2 831-838 bp 1.0 70.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GTGCTCAACCTACGGGAAC 50.0 57.9 58.2 58.2 830-837 bp 1.0 70.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGCTCAACCTACGGGAACT 50.0 52.6 58.2 58.2 829-836 bp 1.0 69.7