Hotspot

matK

LSC · 1932-3449 · 1518 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CTCAGTCGATCAACCTTTAT	TGTTTTCGCTGTATCGCACT	40.0	45.0	52.4	58.2	1714 bp	1.0	40.0
matK_p2	CTCAGTCGATCAACCTTTATT	TGTTTTCGCTGTATCGCACT	38.1	45.0	53.4	58.2	1714 bp	1.0	40.0
matK_p3	CTCAGTCGATCAACCTTTATTT	TGTTTTCGCTGTATCGCACT	36.4	45.0	54.2	58.2	1714 bp	1.0	40.0
matK_p4	CTCAGTCGATCAACCTTTAT	TTGTTTTCGCTGTATCGCAC	40.0	45.0	52.4	57.4	1715 bp	1.0	40.0
matK_p5	CTCAGTCGATCAACCTTTATTTT	TGTTTTCGCTGTATCGCACT	34.8	45.0	55.0	58.2	1714 bp	1.0	40.0