Hotspot

atpF

LSC · 12727-14042 · 1316 bp

Back to Swietenia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1316
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTGCTCAATACGTTCACGGA	AGTCTATCTAAAAGAGGAGATCA	45.0	34.8	57.6	53.5	1452 bp	1.0	40.0
atpF_p2	TTGCTCAATACGTTCACGGA	AAAAAGGTGAAGTGATACAAAA	45.0	27.3	57.6	52.4	1502 bp	1.0	40.0
atpF_p3	TTGCTCAATACGTTCACGGA	AAAAGGTGAAGTGATACAAAAA	45.0	27.3	57.6	52.4	1501 bp	1.0	40.0
atpF_p4	TTGCTCAATACGTTCACGGA	AAAAAGGTGAAGTGATACAAAAA	45.0	26.1	57.6	53.3	1502 bp	1.0	40.0
atpF_p5	TGCTCAATACGTTCACGGAT	AGTCTATCTAAAAGAGGAGATCA	45.0	34.8	57.3	53.5	1451 bp	1.0	40.0