Hotspot

atpA

LSC · 11154-12671 · 1518 bp

Back to Swietenia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	TGTTTGGGGCGATGAAAGAA	50.0	45.0	58.0	58.0	2067 bp	1.0	79.7
atpA_p2	CCTAGCCTTCCAAGCTAACG	TGTTTGGGGCGATGAAAGAA	55.0	45.0	58.1	58.0	2070 bp	1.0	79.4
atpA_p3	GCATCGCGGAATTGAATACG	TGTTTGGGGCGATGAAAGAA	50.0	45.0	58.1	58.0	1971 bp	1.0	79.4
atpA_p4	AGCCTTCCAAGCTAACGATG	GGAGGCAGATCAGTTTCGAG	50.0	55.0	58.0	58.1	2321 bp	1.0	79.2
atpA_p5	AGCCTTCCAAGCTAACGATG	ACGGAAAGTCGAAATGGAGG	50.0	50.0	58.0	57.9	2336 bp	1.0	79.0