Hotspot

matK-trnK-UUU

LSC · 3450-4183 · 734 bp

Back to Swietenia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCCGAAAAAGAAGTGGG	CGTTGTGCAATTCAATCGCT	50.0	45.0	57.7	58.0	915 bp	1.0	77.6
matK-trnK-UUU_p2	CTCCCGAAAAAGAAGTGGGT	CGTTGTGCAATTCAATCGCT	50.0	45.0	57.7	58.0	914 bp	1.0	77.6
matK-trnK-UUU_p3	ACTCCCGAAAAAGAAGTGGG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	836 bp	1.0	76.5
matK-trnK-UUU_p4	CTCCCGAAAAAGAAGTGGGT	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.7	58.2	836 bp	1.0	76.5
matK-trnK-UUU_p5	CTCCCGAAAAAGAAGTGGGT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	835 bp	1.0	76.5