Hotspot

ycf1

SSC · 123054-128537 · 5484 bp

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Pi 0.027

MarkerSeek score 65.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5484
variable_sites: 640
pi: 0.027
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTTCGAACGGTCTTGGAGA	ATCCGGGCAAAATCGATGAA	50.0	45.0	57.9	57.9	971-1031 bp	0.869565	85.2
ycf1_p2	ATTAGGGTGGTTTCGAACGG	ATCCGGGCAAAATCGATGAA	50.0	45.0	57.9	57.9	1014-1031 bp	0.347826	58.1
ycf1_p3	ATTAGGGTGGTTTCGAACGG	CCGGGCAAAATCGATGAAAC	50.0	50.0	57.9	58.1	1012-1029 bp	0.347826	57.7
ycf1_p4	ATTAGGGTGGTTTCGAACGG	GGGCAAAATCGATGAAACGG	50.0	50.0	57.9	58.1	1010-1027 bp	0.347826	57.7
ycf1_p5	TATGGGCCATTCAACAAGGG	ATCCGGGCAAAATCGATGAA	50.0	45.0	57.8	57.9	671-676 bp	0.304348	56.6