Hotspot

psbE-petL

LSC · 64836-65851 · 1016 bp

Back to Swertia

Pi 0.0327

MarkerSeek score 70.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1016
variable_sites: 142
pi: 0.0327
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1607-1850 bp	0.913043	88.2
psbE-petL_p2	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1606-1849 bp	0.913043	88.2
psbE-petL_p3	AAGGATCAAAACGGCCAGTT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	58.0	58.0	1584-1827 bp	0.913043	88.1
psbE-petL_p4	AAAGGATCAAAACGGCCAGT	AAAGGCGCAGTAACAAGGAA	45.0	45.0	58.0	58.0	1729-1972 bp	0.869565	86.2
psbE-petL_p5	AAGGATCAAAACGGCCAGTT	AAAGGCGCAGTAACAAGGAA	45.0	45.0	58.0	58.0	1728-1971 bp	0.869565	86.2