Hotspot

petA-psbJ

LSC · 63225-64054 · 830 bp

Back to Swertia

Pi 0.0287

MarkerSeek score 74.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 830
variable_sites: 118
pi: 0.0287
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGGCTGA	CATATTCCGGATTGGGGTCA	42.9	50.0	57.5	57.1	726-939 bp	0.913043	68.9
petA-psbJ_p2	TGAGAAGGTTCAATTGGCTGA	TGGTTCATATTCCGGATTGGG	42.9	47.6	57.5	57.8	731-938 bp	0.956522	68.4
petA-psbJ_p3	TGAGAAGGTTCAATTGGCTGA	TATTCCGGATTGGGGTCATC	42.9	50.0	57.5	56.8	724-937 bp	0.956522	68.3
petA-psbJ_p4	TGAGAAGGTTCAATTGGCTGA	TCATATTCCGGATTGGGGTC	42.9	50.0	57.5	56.8	727-940 bp	0.956522	68.3
petA-psbJ_p5	TGAGAAGGTTCAATTGGCTGA	GTTCATATTCCGGATTGGGGT	42.9	47.6	57.5	57.8	729-936 bp	0.913043	66.7