Hotspot

ndhC-trnV-UAC

LSC · 50786-51780 · 995 bp

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Pi 0.0314

MarkerSeek score 64.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 104
pi: 0.0314
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCATTCCAATGCACCCTTT	GTCTGATTGTAGTGCCCGTT	45.0	50.0	58.0	57.9	1306-1994 bp	0.913043	87.1
ndhC-trnV-UAC_p2	TGCGCCCATAGCAATAACAT	GTCTGATTGTAGTGCCCGTT	45.0	50.0	58.0	57.9	1680-2368 bp	0.913043	86.5
ndhC-trnV-UAC_p3	TCTCACTAAAGAAGGGGCCA	GTCTGATTGTAGTGCCCGTT	50.0	50.0	58.0	57.9	1632-2320 bp	0.913043	86.4
ndhC-trnV-UAC_p4	ATCCGACTGTCTAGGACTCG	GTCTGATTGTAGTGCCCGTT	55.0	50.0	58.0	57.9	1578-2266 bp	0.913043	86.4
ndhC-trnV-UAC_p5	CCGACTGTCTAGGACTCGAT	GTCTGATTGTAGTGCCCGTT	55.0	50.0	58.0	57.9	1576-2264 bp	0.913043	86.4