Hotspot

psbM-trnD-GUC

LSC · 29542-30403 · 862 bp

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Pi 0.0303

MarkerSeek score 76.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 862
variable_sites: 112
pi: 0.0303
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCGGTAGGAACTAGAATGA	TCCCGGGGATTGTAGTTCAA	47.6	50.0	58.0	58.3	797-1031 bp	1.0	81.0
psbM-trnD-GUC_p2	AGGCGGTAGGAACTAGAATGA	TTCCCGGGGATTGTAGTTCA	47.6	50.0	58.0	58.3	798-1032 bp	1.0	81.0
psbM-trnD-GUC_p3	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	782-1016 bp	1.0	80.0
psbM-trnD-GUC_p4	AGGCGGTAGGAACTAGAATGA	TTTCCCGGGGATTGTAGTTC	47.6	50.0	58.0	57.2	799-1033 bp	1.0	77.4
psbM-trnD-GUC_p5	GGCGGTAGGAACTAGAATGA	TCCCGGGGATTGTAGTTCAA	50.0	50.0	56.4	58.3	796-1030 bp	1.0	76.8