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trnC-GCA-petN

LSC · 27306-28121 · 816 bp

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Pi 0.0226

MarkerSeek score 69.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 86
pi: 0.0226
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	547-873 bp	1.0	89.8
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAACCAGCCCAAGCAAGA	50.0	45.0	58.1	57.6	553-879 bp	1.0	88.4
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TAAACCAGCCCAAGCAAGAC	50.0	50.0	58.1	58.4	552-878 bp	1.0	88.3
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TAGTCCACTTCTTCCCCACA	50.0	50.0	58.1	57.6	606-932 bp	1.0	88.1
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TCCACTTCTTCCCCACACTA	50.0	50.0	58.1	57.6	603-929 bp	1.0	88.0