Hotspot

psaB

LSC · 46516-48720 · 2205 bp

Back to Swartzia

Pi 0.493

MarkerSeek score 80.30

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 958
pi: 0.493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	GTACAAGGACGTGCTGTAGG	CTTCCGGTCGAAAAACAACG	55.0	50.0	58.0	58.0	2650 bp	0.5	59.8
psaB_p2	GTACAAGGACGTGCTGTAGG	AAGGGACCCTCCTATGATCG	55.0	55.0	58.0	58.0	2814 bp	0.5	59.8
psaB_p3	GCGTGGTTATTGGCAAGAAC	CTTCCGGTCGAAAAACAACG	50.0	50.0	58.0	58.0	2744 bp	0.5	59.7
psaB_p4	GTACAAGGACGTGCTGTAGG	AAGGTTATGAGCCTTGCGAG	55.0	50.0	58.0	58.0	2955 bp	0.5	59.7
psaB_p5	GCGTGGTTATTGGCAAGAAC	AAGGGACCCTCCTATGATCG	50.0	55.0	58.0	58.0	2908 bp	0.5	59.7