Hotspot

psaA

LSC · 44238-46490 · 2253 bp

Back to Swartzia

Pi 0.483

MarkerSeek score 79.00

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: LSC
length_bp: 2253
variable_sites: 916
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	CTAATGGCTGCTCACGTGAT	CCCGGAAGTCCACAGAAAAA	50.0	50.0	58.1	58.0	2879 bp	0.5	59.4
psaA_p2	GCGGGTCTCTTTGTATGTGT	CCCGGAAGTCCACAGAAAAA	50.0	50.0	57.9	58.0	2509 bp	0.5	59.0
psaA_p3	GCGGGTCTCTTTGTATGTGT	CCCCCTCGAGTAAAAGCTTC	50.0	55.0	57.9	58.0	2643 bp	0.5	59.0
psaA_p4	GCGGGTCTCTTTGTATGTGT	TCGGTTTCCATTTCGGTTGT	50.0	45.0	57.9	58.0	2810 bp	0.5	59.0
psaA_p5	AAATACCGGTATGTCCCCCA	CCCGGAAGTCCACAGAAAAA	50.0	50.0	58.1	58.0	2905 bp	0.5	59.0