Hotspot

rpl32-trnL-UAG

SSC · 118556-119378 · 823 bp

Back to Swartzia

Pi 0.0691

MarkerSeek score 81.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 823
variable_sites: 52
pi: 0.0691
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGCAACAAATACAAACGTTGGA	TCCTAAGAGCAGCGTGTCTA	36.4	50.0	57.6	57.9	888-901 bp	1.0	68.7
rpl32-trnL-UAG_p2	GTGCAACAAATACAAACGTTGG	TCCTAAGAGCAGCGTGTCTA	40.9	50.0	57.6	57.9	889-902 bp	1.0	68.2
rpl32-trnL-UAG_p3	TGCAACAAATACAAACGTTGGA	AGCAGCGTGTCTACCAATTT	36.4	45.0	57.6	57.8	881-894 bp	1.0	68.2
rpl32-trnL-UAG_p4	TGCAACAAATACAAACGTTGGA	CCTAAGAGCAGCGTGTCTAC	36.4	55.0	57.6	57.8	887-900 bp	1.0	68.1
rpl32-trnL-UAG_p5	TGCAACAAATACAAACGTTGGA	CTAAGAGCAGCGTGTCTACC	36.4	55.0	57.6	57.8	886-899 bp	1.0	68.1