Hotspot

psbZ-trnT-UGU

LSC · 39209-39934 · 726 bp

Back to Swartzia

Pi 0.257

MarkerSeek score 81.70

Primer available yes

Label psbZ-trnT-UGU

Feature Metadata

label_name: psbZ-trnT-UGU
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 164
pi: 0.257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnT-UGU_p1	CTTCTCCTGATGGTTGGTCG	AAAATCTGACCGGTTGACCC	55.0	50.0	58.0	58.1	1548 bp	0.5	59.1
psbZ-trnT-UGU_p2	CTTCTCCTGATGGTTGGTCG	CCGGTTGACCCTTTAGCTTT	55.0	50.0	58.0	58.1	1539 bp	0.5	59.1
psbZ-trnT-UGU_p3	CTTCTCCTGATGGTTGGTCG	AGCGCGATTGGTTTCTAAGT	55.0	45.0	58.0	57.9	1412 bp	0.5	59.1
psbZ-trnT-UGU_p4	CTTCTCCTGATGGTTGGTCG	CTGACCGGTTGACCCTTTAG	55.0	55.0	58.0	57.9	1543 bp	0.5	59.1
psbZ-trnT-UGU_p5	TCGATCCATGCCTCAAAAGG	CCGGTTGACCCTTTAGCTTT	50.0	50.0	58.0	58.1	1793 bp	0.5	58.9