Hotspot

trnK-UUU-rps16

LSC · 4262-5128 · 867 bp

Back to Swartzia

Pi 0.0679

MarkerSeek score 78.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 53
pi: 0.0679
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCTGGATCAGTCGTGG	GGTTGCGAATACGAGGAGTT	55.0	50.0	58.0	58.0	1449-1451 bp	1.0	87.2
trnK-UUU-rps16_p2	CCTTTCTGGATCAGTCGTGG	AAGACAGGCAAGAAAAGGGG	55.0	50.0	58.0	58.0	1546-1553 bp	1.0	87.0
trnK-UUU-rps16_p3	AGCTTTTCCCACATCAAGCA	GGTTGCGAATACGAGGAGTT	45.0	50.0	58.0	58.0	1708-1711 bp	1.0	86.9
trnK-UUU-rps16_p4	AGCTTTTCCCACATCAAGCA	AAGACAGGCAAGAAAAGGGG	45.0	50.0	58.0	58.0	1805-1813 bp	1.0	86.8
trnK-UUU-rps16_p5	AGCTTTTCCCACATCAAGCA	AAAAGGGGGTAGAGACTGCT	45.0	50.0	58.0	58.0	1793-1801 bp	1.0	86.5