Hotspot

ndhF-rpl32

SSC · 110562-111478 · 917 bp

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Pi 0.0179
MarkerSeek score 57.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
917
variable_sites
16
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCTGCAATAGGTCGGGTAAA AAGAACTTGTTGATTGCGCT 50.0 40.0 58.0 56.5 1822-1833 bp 1.0 70.2
ndhF-rpl32_p2 GCTGCAATAGGTCGGGTAAA AGAACTTGTTGATTGCGCTT 50.0 40.0 58.0 56.5 1821-1832 bp 1.0 70.2
ndhF-rpl32_p3 AAAAGTCCACATACGCCGAA AAGAACTTGTTGATTGCGCT 45.0 40.0 57.8 56.5 1433-1444 bp 1.0 69.5
ndhF-rpl32_p4 AAAAGTCCACATACGCCGAA AGAACTTGTTGATTGCGCTT 45.0 40.0 57.8 56.5 1432-1443 bp 1.0 69.5
ndhF-rpl32_p5 AAAGTCCACATACGCCGAAG AGAACTTGTTGATTGCGCTT 50.0 40.0 58.3 56.5 1431-1442 bp 1.0 68.7