Hotspot

trnG-trnR

LSC · 8606-8758 · 153 bp

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Pi 0.028

MarkerSeek score 67.20

Primer available yes

Label trnG-trnR

Feature Metadata

label_name: trnG-trnR
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 4
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnR_p1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	265-275 bp	1.0	51.3
trnG-trnR_p2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	271-281 bp	1.0	51.2
trnG-trnR_p3	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.9	53.9	285-295 bp	1.0	50.8
trnG-trnR_p4	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	273-283 bp	1.0	50.8
trnG-trnR_p5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	266-276 bp	1.0	50.3