Hotspot

trnS-trnG

LSC · 7190-7811 · 622 bp

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Pi 0.0322

MarkerSeek score 75.10

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 622
variable_sites: 20
pi: 0.0322
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	CCAACGCTTTAGTCCACTCA	AATCAAACCGAGAGACCCTT	50.0	45.0	57.8	56.5	730-804 bp	1.0	68.5
trnS-trnG_p2	CCAACGCTTTAGTCCACTCA	ATCAAACCGAGAGACCCTTT	50.0	45.0	57.8	56.5	729-803 bp	1.0	68.5
trnS-trnG_p3	AACGCTTTAGTCCACTCAGC	AATCAAACCGAGAGACCCTT	50.0	45.0	58.2	56.5	728-802 bp	1.0	68.2
trnS-trnG_p4	AACGCTTTAGTCCACTCAGC	ATCAAACCGAGAGACCCTTT	50.0	45.0	58.2	56.5	727-801 bp	1.0	68.2
trnS-trnG_p5	CCAACGCTTTAGTCCACTCA	AATCAAACCGAGAGACCCTTT	50.0	42.9	57.8	57.2	730-804 bp	1.0	66.6