Hotspot

ycf1

SSC · 117675-123950 · 6276 bp

Back to Suaeda

Pi 0.104
MarkerSeek score 73.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
6276
variable_sites
1777
pi
0.104
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.85
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CTGGTGGCTTCTCTTCTTCC GTGCCACGTTAGCCAGATTA 55.0 50.0 57.9 58.0 1047-1095 bp 0.2 51.4
ycf1_p2 CTGGTGGCTTCTCTTCTTCC TTTTCCCAAGTGCCACGTTA 55.0 45.0 57.9 57.9 1056-1104 bp 0.2 51.1
ycf1_p3 GCTTAGCAACTGGTGGCTTA GTGCCACGTTAGCCAGATTA 50.0 50.0 58.2 58.0 1209-1257 bp 0.2 50.2
ycf1_p4 CTGGTGGCTTCTCTTCTTCC GAGCTACTGAGGAACAACGG 55.0 55.0 57.9 58.0 1919-1967 bp 0.2 49.6
ycf1_p5 TTACCGCCTTTTCGAATCGA GAACAAGATAAGCCTGCGGA 45.0 50.0 57.6 58.0 415 bp 0.2 45.1