Hotspot

rps16

LSC · 4433-5558 · 1126 bp

Back to Suaeda

Pi 0.064
MarkerSeek score 81.10
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1126
variable_sites
204
pi
0.064
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 CCATTGAGTTAGCAACCCGA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 2269-2454 bp 0.9 86.5
rps16_p2 CCATTGAGTTAGCAACCCGA TGGGCTGAAATTGACGAACA 50.0 45.0 57.9 58.0 2383-2568 bp 0.8 82.9
rps16_p3 CCATTGAGTTAGCAACCCGA ATAGCAATTTAGGTGGGGCG 50.0 50.0 57.9 58.0 2330-2515 bp 0.7 79.0
rps16_p4 ACCATTGAGTTAGCAACCCG ATAGCAATTTAGGTGGGGCG 50.0 50.0 58.2 58.0 2331-2516 bp 0.7 78.4
rps16_p5 CCATTGAGTTAGCAACCCGA GAATCCTTCCGTCCCAGAAC 50.0 55.0 57.9 58.0 2262-2367 bp 0.5 70.9