Hotspot

trnN-GUU-ndhF

IRb · 101727-103964 · 2238 bp

Back to Suaeda

Pi 0.144
MarkerSeek score 72.70
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
2238
variable_sites
719
pi
0.144
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.64
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 CACATTCTGGTGTCCTAGGC AAACGGGTTTGGTGCTGTAA 55.0 45.0 58.0 58.2 2923 bp 0.2 46.0
trnN-GUU-ndhF_p2 GGTGACGATACTGTAGGGGA AAACGGGTTTGGTGCTGTAA 55.0 45.0 57.9 58.2 2852 bp 0.2 45.7
trnN-GUU-ndhF_p3 ACGCCAGAAAGGTTCCTTTT AAACGGGTTTGGTGCTGTAA 45.0 45.0 57.9 58.2 2810 bp 0.2 45.7
trnN-GUU-ndhF_p4 GAACTTGGGTGTGGGTCTTT AAACGGGTTTGGTGCTGTAA 50.0 45.0 57.9 58.2 2601 bp 0.2 45.7
trnN-GUU-ndhF_p5 AATAGCTCGACGCCAGAAAG AAACGGGTTTGGTGCTGTAA 50.0 45.0 58.1 58.2 2819 bp 0.2 45.6