Hotspot

psbM-trnD-GUC

LSC · 28166-28878 · 713 bp

Back to Suaeda

Pi 0.12
MarkerSeek score 79.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
713
variable_sites
259
pi
0.12
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 CTAGCCTGCTCGTTTCAGTT GTCTTCCCGAAATGGCTGAT 50.0 50.0 57.9 58.0 1404-1425 bp 0.4 62.8
psbM-trnD-GUC_p2 TCGAGCATCTATCCACCAGT GTCTTCCCGAAATGGCTGAT 50.0 50.0 57.9 58.0 1819-1886 bp 0.3 58.4
psbM-trnD-GUC_p3 TCGAGCATCTATCCACCAGT TTCCCGAAATGGCTGATACG 50.0 50.0 57.9 58.1 1816-1883 bp 0.3 58.3
psbM-trnD-GUC_p4 CTAGCCTGCTCGTTTCAGTT TTCCCGAAATGGCTGATACG 50.0 50.0 57.9 58.1 1410-1422 bp 0.3 57.9
psbM-trnD-GUC_p5 TCGAGCATCTATCCACCAGT GATTCTTCCTGGGTTGGGTC 50.0 55.0 57.9 57.9 1958 bp 0.2 46.4