Hotspot

trnC-GCA-petN

LSC · 26864-27355 · 492 bp

Back to Suaeda

Pi 0.0945
MarkerSeek score 83.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
492
variable_sites
126
pi
0.0945
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 571-622 bp 1.0 88.5
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG GCCCAAGCGAGACTTACAAT 50.0 50.0 58.1 58.3 509-558 bp 0.9 85.3
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG ATTAAAGCAGCCCAAGCGAG 50.0 50.0 58.1 58.9 518-569 bp 1.0 84.1
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG GACTACCATTAAAGCAGCCCA 50.0 47.6 58.1 58.0 525-576 bp 1.0 83.3
trnC-GCA-petN_p5 CCAGTTCAAATCCGGGTGTC GCCCAAGCGAGACTTACAAT 55.0 50.0 58.8 58.3 504-553 bp 0.9 79.2