Hotspot

atpH-atpI

LSC · 12828-13561 · 734 bp

Back to Suaeda

Pi 0.0872
MarkerSeek score 80.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
734
variable_sites
181
pi
0.0872
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 GCCAGTGAACGAAGAAAACG GGGGCACTTTTACCTTGGAA 50.0 50.0 58.0 58.0 1837-1879 bp 0.9 87.8
atpH-atpI_p2 GCCAGTGAACGAAGAAAACG TTGGTCAGGGGCACTTTTAC 50.0 50.0 58.0 58.0 1844-1886 bp 0.9 87.8
atpH-atpI_p3 ATAACGGAAGCGGCAGAAAT GGGGCACTTTTACCTTGGAA 45.0 50.0 58.0 58.0 1137-1178 bp 0.9 87.7
atpH-atpI_p4 CCAGTGAACGAAGAAAACGC GGGGCACTTTTACCTTGGAA 50.0 50.0 58.0 58.0 1836-1878 bp 0.8 83.6
atpH-atpI_p5 CCAGTGAACGAAGAAAACGC TTGGTCAGGGGCACTTTTAC 50.0 50.0 58.0 58.0 1843-1885 bp 0.8 83.5