Hotspot

matK-trnK-UUU

LSC · 3029-3762 · 734 bp

Back to Suaeda

Pi 0.0695
MarkerSeek score 81.70
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
734
variable_sites
141
pi
0.0695
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 AGTTCTATATGTCTTTGGAATTCT GGTAGAGTACTCGGCTTTTA 29.2 45.0 53.5 53.9 751-784 bp 1.0 52.0
matK-trnK-UUU_p2 AGTTCTATATGTCTTTGGAATTCTTC GGTAGAGTACTCGGCTTTTA 30.8 45.0 55.4 53.9 751-784 bp 1.0 52.0
matK-trnK-UUU_p3 AGTTCTATATGTCTTTGGAATTCT GGTAGAGTACTCGGCTTTTAA 29.2 42.9 53.5 54.8 751-784 bp 1.0 52.0
matK-trnK-UUU_p4 AGTTCTATATGTCTTTGGAATTCTT GGTAGAGTACTCGGCTTTTA 28.0 45.0 54.3 53.9 751-784 bp 1.0 52.0
matK-trnK-UUU_p5 AGTTCTATATGTCTTTGGAATTCTTC GGTAGAGTACTCGGCTTTTAA 30.8 42.9 55.4 54.8 751-784 bp 1.0 52.0