Hotspot

ycf1

SSC · 127509-133169 · 5661 bp

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Pi 0.00865
MarkerSeek score 48.00
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5661
variable_sites
280
pi
0.00865
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ATCGATCTTGATCCAGGCCT ATCCGTCTTGTCACAAGCAT 50.0 45.0 58.3 57.5 724-739 bp 1.0 82.0
ycf1_p2 ATCGATCTTGATCCAGGCCT TATCCGTCTTGTCACAAGCA 50.0 45.0 58.3 56.9 725-740 bp 1.0 76.8
ycf1_p3 ATCGATCTTGATCCAGGCCT TCCGTCTTGTCACAAGCATA 50.0 45.0 58.3 56.9 723-738 bp 1.0 76.8
ycf1_p4 TCGATCTTGATCCAGGCCTC ATCCGTCTTGTCACAAGCAT 55.0 45.0 59.0 57.5 723-738 bp 1.0 76.5
ycf1_p5 GATCTTGATCCAGGCCTCAA ATCCGTCTTGTCACAAGCAT 50.0 45.0 57.0 57.5 721-736 bp 1.0 76.0