Hotspot

ndhF-rpl32

SSC · 115968-116789 · 822 bp

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Pi 0.00916
MarkerSeek score 64.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
822
variable_sites
42
pi
0.00916
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAAAAGGTATGATCCACGCA AGCTTTTAAGGCTGCCCAAT 42.9 45.0 57.9 58.1 935-973 bp 1.0 77.4
ndhF-rpl32_p2 ACAAAAGGTATGATCCACGCA AAAGCTTTTAAGGCTGCCCA 42.9 45.0 57.9 58.3 937-975 bp 1.0 75.5
ndhF-rpl32_p3 ACAAAAGGTATGATCCACGCA AAGCTTTTAAGGCTGCCCAA 42.9 45.0 57.9 58.3 936-974 bp 1.0 75.5
ndhF-rpl32_p4 ACAAAAGGTATGATCCACGCA AGGCTGCCCAATATCCTTTC 42.9 50.0 57.9 57.6 927-965 bp 1.0 74.8
ndhF-rpl32_p5 ACAAAAGGTATGATCCACGCA GCTGCCCAATATCCTTTCCT 42.9 50.0 57.9 57.6 925-963 bp 0.962963 73.2