Hotspot

petA-psbJ

LSC · 66141-67187 · 1047 bp

Back to Styrax

Pi 0.0149
MarkerSeek score 75.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1047
variable_sites
70
pi
0.0149
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCATCTGTTGTTTTGGCACA ATTCCGGATTGGGTTCATCC 45.0 50.0 57.8 57.6 940-1193 bp 1.0 84.3
petA-psbJ_p2 AGCATCTGTTGTTTTGGCAC ATTCCGGATTGGGTTCATCC 45.0 50.0 57.5 57.6 941-1194 bp 1.0 82.0
petA-psbJ_p3 GCATCTGTTGTTTTGGCACA TGGTTCATATTCCGGATTGGG 45.0 47.6 57.8 57.8 948-1201 bp 1.0 77.5
petA-psbJ_p4 GCATCTGTTGTTTTGGCACA GGTTCATATTCCGGATTGGGT 45.0 47.6 57.8 57.8 947-1200 bp 1.0 77.4
petA-psbJ_p5 CGAGAAGGTTCAATTGTCCG ATTCCGGATTGGGTTCATCC 50.0 50.0 56.8 57.6 890-1143 bp 1.0 77.1