Hotspot

trnC-GCA-petN

LSC · 28944-29691 · 748 bp

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Pi 0.00958
MarkerSeek score 60.70
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
748
variable_sites
45
pi
0.00958
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GGGGGACTGCAAATCCTTTT CAGCCCAAGCGAGATTTACT 50.0 50.0 58.4 58.0 811-875 bp 1.0 85.2
trnC-GCA-petN_p2 AGTTCAAATCTGGGTGTCGC CAGCCCAAGCGAGATTTACT 50.0 50.0 58.5 58.0 786-850 bp 1.0 84.4
trnC-GCA-petN_p3 TATCCTTTTGGCGACATGGC CAGCCCAAGCGAGATTTACT 50.0 50.0 58.6 58.0 844-908 bp 1.0 83.0
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.4 57.6 871-935 bp 1.0 82.4
trnC-GCA-petN_p5 AGTTCAAATCTGGGTGTCGC AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.5 57.6 846-910 bp 1.0 81.5