Hotspot

trnK-UUU-rps16

LSC · 4338-4942 · 605 bp

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Pi 0.0126
MarkerSeek score 72.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
605
variable_sites
42
pi
0.0126
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GGACGAATCCCTTGCTTCAT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 765-800 bp 1.0 78.2
trnK-UUU-rps16_p2 ATGGACGAATCCCTTGCTTC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 767-802 bp 1.0 78.1
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC GGTGCTCAACCTACAGAAACT 55.0 47.6 57.9 57.9 774-809 bp 1.0 77.8
trnK-UUU-rps16_p4 CGAATCCCTTGCTTCATCCA GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 762-797 bp 0.925926 74.8
trnK-UUU-rps16_p5 ACGAATCCCTTGCTTCATCC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 763-798 bp 0.925926 74.8