Hotspot

clpP

LSC · 74155-76182 · 2028 bp

Back to Styphnolobium

Pi 0.00123

MarkerSeek score 49.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2028
variable_sites: 5
pi: 0.00123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TATTTGCGCGGAATTCTTGC	ACCGATTGTTACGTTTCCACA	45.0	42.9	57.8	58.2	2116-2118 bp	1.0	69.5
clpP_p2	CGTGTATTTGCGCGGAATTC	ACCGATTGTTACGTTTCCACA	50.0	42.9	58.5	58.2	2120-2122 bp	1.0	66.6
clpP_p3	TATTTGCGCGGAATTCTTGC	CCGATTGTTACGTTTCCACA	45.0	45.0	57.8	56.4	2115-2117 bp	1.0	65.8
clpP_p4	TATTTGCGCGGAATTCTTGC	ACCGATTGTTACGTTTCCAC	45.0	45.0	57.8	56.4	2116-2118 bp	1.0	65.8
clpP_p5	ATCGTGTATTTGCGCGGAAT	ACCGATTGTTACGTTTCCACA	45.0	42.9	58.7	58.2	2122-2124 bp	1.0	65.3